• UNIT 16 ASEXUAL REPRODUCTION IN PLANTS

    Key Unit Competence
    Account for various methods of asexual reproduction as means of increasing crop yield.

    Learning Objectives
    By the end of this unit, I should be able to:
    –– Describe the various methods of asexual reproduction: fragmentation, budding, and spore formation.
    –– Demonstrate asexual reproduction mechanisms in lower organisms.
    –– Discuss the advantages and disadvantages of asexual reproduction.
    –– Describe the characteristics of vegetative reproductive parts in a flowering plant.
    –– Apply principles of artificial propagation in growing varieties of plants that are economically important.
    –– Apply the knowledge acquired to produce economically important plants.
    –– Appreciate the use of artificial propagation in increasing crop yields.
    Introductory activity
    The kingdom Plantae comprises about 260,000 known species including flowering
    and non-flowering plants. All plants have a general organization which includes
    vegetative and reproductive organs. Plants reproduce through different ways:
    Use the books and other source of information to
    1. Write on how lower organisms such unicellular plant and another like cassava, sugar cane and apple reproduce.
    2. Describe the techniques used by people to grow Irish potatoes, cassava and bananas.
    3. Describe each of the following methods of asexual reproduction:
    fragmentation, budding and spore formation.

    16.1 Asexual reproduction
    Activity 16.1
    Using textbooks or internet and search on asexual reproduction.

    Asexual Reproduction
    This is a type of reproduction without production of gametes. It usually results in the production of identical offspring; the genetic variation arises as a result of random mutation among the individuals.

    Self-assessment 16.1
    Write an essay on asexual reproduction.

    16.2 Types of asexual reproduction
    Activity 16.2.1
    Discuss on asexual reproduction in lower organisms and write a report as an outof-class activity.
    There are five common modes of asexual reproduction: fission, budding, vegetative reproduction, spore formation and fragmentation.

    16.2.1 Fission
    An important form of fission is binary fission. In binary fission, the parent organism is replaced by two daughter organisms, because it divides in two, i. e: Unicellular Fungi. Another type of fission is multiple fission that occurs in many algae. The nucleus of the parent cell divides several times by mitosis, producing several nuclei. The cytoplasm then separates, creating multiple daughter cells.

    16.2.2 Budding
    Some cells split via budding resulting in a ‘mother’ and ‘daughter’ cell. The offspring organism is smaller than the parent. Budding is also known on a multicellular level.

    16.2.3 Vegetative reproduction
    Vegetative reproduction is a type of asexual reproduction found in plants where new individuals are formed without the production of seeds or spores. Examples of vegetative reproduction include the formation of miniaturized plants called plantlets on specialized leaves (for example in kalanchoe) and some produce new plants out of rhizomes or stolon (for example in strawberry). Other plants reproduce by forming bulbs or tubers (for example tulip bulbs and dahlia tubers). Some plants produce adventitious shoots and suckers that form along their lateral roots. Vegetative reproduction may form a clonal colony where all the individuals are clones.

    Activity16.2.2
    Demonstration of asexual reproduction by fragmentation in algae

    Requirements:
    Glass beakers of 500ml, Scalpel, Forceps, Pins, Spatula, Weighing balance, Labels,
    Artificial, fertilizers, Clear river water and Spirogyra (algae).

    Procedure
    1. Label five beakers of the same size as A, B, C, D and E. Pour water in each
    beaker. Weigh several measures of artificial fertilizers of 1 g each.
    2. Transfer 1g of fertilizer to beaker A, then 2g to beaker B, 3g to beaker C,
    4g to beaker D and 5g of fertilizers to beaker E. Note the concentration of
    fertilizers is increasing from A- E.
    3. Using forceps pick spirogyra and put it on a tile. Add several drops of
    water to avoid drying. Tease off a piece of spirogyra using a pin. Cut that
    piece into 5 fragments of the same length and transfer each piece into the
    beaker.
    4. Stand the beakers in a place where they can receive adequate sunlight for
    the seven days. On the next day, start to examine the fragments in each
    beaker every day and record any observable changes such the increase in
    size of the spirogyra.
    Draw a table as this shown here and record your observation.

    16.2.5 Fragmentation
    Fragmentation is a form of asexual reproduction where a new organism grows from a fragment of the parent. The fragment develops into a mature and fully grown individual. Fragmentation is seen in many organisms such as fungiplants and gemma in liverworts. Most lichens, which are a symbiotic union of a fungus and photosynthetic algae, reproduce through fragmentation to ensure that new individuals contain both symbionts. These fragments can take the form of soredia, dust-like particles consisting of fungal hyphen wrapped around photobiont cells.


    16.3 Advantages and disadvantages of asexual reproduction
    Self-assessment 16.2

    1. Write a short essay on asexual reproduction.
    2. What is itfragmentation? give one example of plant which reproduces by fragmentation

    16.3.1 Advantages of asexual reproduction.
    Activity 16.3

    Discuss on asexual reproduction in lower organisms and higher plants, and outlining advantages and disadvantages.

    Since asexual reproduction does not involve the process of gamete formation. It can be completed much more quickly, allowing the individual to spread its genetic material in a shorter period of time. It is also an advantage for small populations. as there may not be an adequate number of plants to pollinate one another to reproduce. If the parent plants are successful, it is a good idea to make clones of the plant that can be successful as well.

    16.3.2 Disadvantages of asexual reproduction.
    Asexual reproduction does not have genetic diversity. With sexual reproduction, mixing a gene pool can produce diversity. The organism becomes less adapted to certain environmental changes when there is less variation produced in offspring. The entire communities of the genetically identical organisms might not survive such changes. Asexual reproduction usually leads to struggle for existence as well as overcrowding. The main distinction of asexual reproduction from sexual reproduction is that there is no need for two parents as well as special cells to reproduce. It means that special the mechanisms of combining sex cells for fertilization is no longer required. This form of reproduction process is simply mitosis that copy a parent organism.

    Self-assessment 16.3
    Explain the advantages and disadvantages of asexual reproduction.

    16.4 Vegetative and artificial propagation in flowering plants
    Activity 16.4
    Demonstration of asexual reproduction in plants by cuttings

    Requirements
    Growth medium or moist soil, sweet potatoes vines, elephant grass, sugarcane or cassava stems, secateurs/sharp knife and rooting hormone.

    Procedure
    1. Collect clean and healthy stems from cassava, sugarcane or potato plants.
    2. Using a secateurs/sharp knife, cut the stem of either cassava, sugarcane or
    sweet potato stems into fragments of suitable sizes.
    3. Place them in either suitable medium of growth with rooting hormone if
    available or plant them in moist soil in the school garden.
    4. Leave the set up for about 13 days, and then observe the development of
    roots and leaves at nodes.
    Draw and record what you will observe after 13 days on the development of roots
    and leaves at nodes.

    Artificial vegetative propagation is the deliberate production of new plants from parts of old plants. This can be done by following three methods: Cutting, layering, and grafting.

    a. Cutting
    This is a simple procedure in which part of the plant called a ‘cutting’is removed from the parent plant by cutting and placed in a suitable medium for grow. In this method, one-year-old stem of root is cut from a distance of 20 to 30 cm and buried in the moist soil in natural position. After sometime, roots develop from this cutting and it grows into a new plant. This method is commonly used in rose and sugar cane. Care is taken that nodes which were lower in parent plant (morphologically) are put in the soil, while the morphologically higher nodes are kept up. Adventitious roots are given off at the lower nodes.

    b. Layering 
    This method of vegetative propagation is used in those plants with soft branches such as jasmine plant. In this method, a branch of the plant which is near to the ground is pulled towards the ground and covered with moist soil leaving the tip of this branch above the ground. After sometime, roots develop from that part of the branch which was buried in the soil. This branch is then cut of along with the roots from the parent plant and develops into a new plant. This method of asexual reproduction is also used in the production of plants such as Bougainvillea, guava, strawberries, lemon, China rose etc.
    c. Grafting
    In this method of vegetative propagation, the stems of two diff erent plants are joined together so as to produce a new plant containing the characters of both plants. The plant of which the root system is taken is called ‘stock’, while the other plant of which the shoot is selected is known as ‘scion’ or ‘graft’. These two stems i.e. the stock and the scion are fi tted together by making slanting cuts into them and bound tightly with a piece of cloth and then covering it with a polythene sheet.
    The diameter of the stock and scion chosen for grafting should be equal. Scion gets
    the mineral and water from the soil through the stock and develops branches and produce fruits. This method of propagation is used in mango, apple, banana, pear, grape, pineapple and peach.
    Vegetative reproductive parts in fl owering plants
    The reproductive part of the plant is a fl ower. The union of male and female gametes to form a zygote is called fertilization. The transfer of pollen grains from the anther to the stigma of the same fl ower or the diff erent fl ower is called pollination. Plants reproduce asexually in a variety of ways. The vegetative reproductive parts in fl owering plant are stem, branches, and leaves and they have the following characteristics:

      Characteristics of Stem:
     Stem develops from the plumule of embryo. It is generally the ascending part of the plant axis.It bears a terminal bud for growth in length and is diff er entiated into nodes and internodes. The stem nodes possess dissimilar appendages called leaves. The young stem is green and capable of performing photosynthesis. In the mature state, stem bears fl owers and fruits. Leaves and stem branches develop exog enously. Stem exposes leaves, fl owers and fruits to their most suitable position in the aerial environment for optimum function. Stems usually show positively phototropic, negatively geotropic and negatively hydrotropic.

    Characteristics of Leaf
    – It is dissimilar lateral fl attened outgrowth of the stem,
    – The leaf is exogenous in origin
    – It is borne on the stem in the region of a node,
    – An axillary bud is often present in the axil of the leaf.
    – Leaf has limited growth. An apical bud or a regular growing point is absent,
    – The leaf base may possess two lateral outgrowths called stipules,
    –– A leaf is differentiated into three parts: leaf base, petiole and lamina.
    –– The lamina possesses prominent vascular strands called veins,
    –– It is green and specialized to perform photosynthesis,
    –– Leaf bears abundant stomata for exchange of gases and it is the major seat of
    transpiration.

      Characteristics of branches
    A branch a woody structural member connected to but not part of the central trunk of a tree. Large branches are known as boughs and small branches are known as twigs. Due to a broad range of species of trees, branches and twigs can be found in many different shapes and sizes.

     Self-assessment 16.4

    1. Discuss on the methods of artificial vegetative propagation.
    2. Cassava produces flowers, fruits and seeds. Why people prefer to grow
    cassava by cutting rather than using seed?
    3. Describe the characteristics of vegetative reproductive parts in a flowering
    plant

    16. 5 Application of artificial propagation in production of improved varieties of plants
    Activity 16.5
    Artificial vegetative propagation is usually used in agriculture for the propagation (or reproduction) of those plants which produce either very few seeds or do not produce viable seeds. Such plants include: Banana, Pineapple, Orange, Grape, Rose, etc.

    Reasons for preferring vegetative rather than sexual means of reproduction vary, but commonly include greater ease and speed of propagation of certain plants. Another major attraction is that the resulting plants are clones of the parent plants.

    The methods of vegetative reproduction range from rooting cuttings to grafting and artificial propagation by laboratory tissue culture. In horticulture, a “cutting” is a piece that has been cut off from a mother plant and then caused to grow into a whole plant. A popular use of grafting is to produce fruit trees, sometimes with more than one variety of the same fruit species growing from the same stem. Rootstocks for fruit trees are either seedlings or propagated by layering.

    Self-assessment 16.5

    Explain the application of artificial propagation in production of improved varieties of plants.

    End of unit assessment16
    A. Multiple choice questions: choose the best answers.
    1. In vegetative propagation, cuttings are mainly taken from
    a. Leaves of parent plant
    b. Roots or stems of parent plant
    c. Shoots of parent plant
    d. Buds of parent plant
    2. Artificial methods of vegetative propagation includes
    a. Cloning
    b. Grafting
    c. Cuttings
    d. Both b and c
    3. Example of plant in which vegetative propagation is occurred by leaves is
    called
    a. Cannabis
    b. Chrysanthemum
    c. Bryophyllum
    d. Brassica
    4. Which of the following is NOT an advantage of asexual reproduction?
    a. Rapid reproduction.
    b. High genetic diversity.
    c. No need for a mate.
    d. Low resource investment in offspring.

    B.Questions with short and long answers
    1. Name the plants which are grown by grafting method.
    2. What do you understand by grafting?
    3. Show how vegetative propagation takes place in potatoes?
    4. Explain the method by which the sugarcane and rose are propagated.
    5. Give the names of the different methods of artificial vegetative reproduction.
    6. Explain the term vegetative reproduction and give one example of plant
    which reproduces by using this type of asexual reproduction.





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