Key Unit Competence
    Explain how diversity is threatened by climate change and human activities

    Learning objectives
    By the end of this unit, I should be able to:
    –– Define the terms: species, ecosystem and niche.
    –– Explain that biodiversity is considered at three different levels
    –– Evaluate the consequences of loss of biodiversity.
    –– Characterize the biotic and abiotic components that define Rwanda’s ecosystems (example: freshwater, marine, and terrestrial).
    –– Apply Simpson’s Index of Diversity.
    –– Explain the importance of random sampling in determining the biodiversity of an area.
    –– Use suitable survey methods such as frame quadrats, line and belt transects to assess the distribution and abundance of organisms in a local area.
    –– Use Pearson’s linear correlation to analyze the relationships between the
    distribution and abundance of species and abiotic or biotic factors.
    –– Recognize that the biodiversity of the earth is threatened by human activities and climate change

    Introductory activity: Biodiversity of Rwanda

    Read the following text and answer the questions that follow

    Rwanda is located at the heart of the Albertine Rift eco-region in the western arm of the Africa’s Rift Valley. Habitats of Rwanda are equally varied, ranging from Afro-Montana ecosystems in the northern and western regions to lowland forests, savannah woodlands and savannah grasslands in the southern and eastern regions. There are other habitats around volcanic hot springs and old lava flows, especially in the northern and western part of the country.

    Rwanda also has several lakes and wetlands which are rich in different species. Though not yet well surveyed, all these ecosystems host a rich variety of fauna and flora and micro-organisms. This rich biodiversity is mainly conserved in protected areas including three national parks, natural forests and wetlands. These cover almost 10 percent of the national territory while the rest of the country is densely populated (507 people per square kilometer in 2018).

    Many tourists visit Rwanda for its beautiful environment and biodiversity made of different species of plants and animals such as Aloe vera (Igikakarubamba), Muringa oleifera (Muringa), Phaseolus vulgaris (common bean), Nymphaea thermarum (Endemic plant species that cannot be met elsewhere in the world, only found in Mashyuza minor locality harbors),
    Colobus polykoma (White-black colobus monkey), Gorilla gorilla (mountain
    gorilla) bird Laniarius mufumbiri (Bird species mainly found in Rweru- Mugera wetland),etc.

    The most attracting species in Rwanda is Gorilla gorilla whose habitat is the mountains of Birunga where they make a large population. Another natural forest, Nyugwe National Park is a terrestrial ecosystem that contains a large community of different plants and animals.

    Rwanda also has different lakes such as Muhazi and Rumira. They are aquatic ecosystems made of few species of fish, such as tilapias. Tilapias from Lake Muhazi are small, black and bony fish while those from Lake Rumira look red, big and soft. Tilapias from both lakes still belong in the same species but show variations.

    Many species of animals and plants have been discovered in Rwanda but some species also disappeared. Today the big garden snails known as Achatina achatina have become rare in Bugesera. Other people poached Rhinoceros alba living in Savanah of Akagera National Park.

    Honey bees, butterflies and grasshoppers are small in size but still important for different ecosystem services. Each organism is important for its niche in ecosystem. We need to identify and protect the biodiversity of our ecosystem. Many tourists enjoy visiting Rwanda for its biodiversity.
    1. Name the species not found elsewhere that attract the tourists and locate where it is found.
    2. Mashyuza is a minor locality in western province in Rusizi district that contributes to biodiversity of Rwanda. Give any other two locations.
    3. Define each of the following biological terms and give an example from the text
    above:(a) Species (b) Population (c) Community (d) Habitat (e) Ecosystem
    (f )Variation (g) Niche
    4. What causes some species to become extinct?
    5. What can be the consequences of the loss of some species from our
    6. Do you support tourism in Rwanda? Give a reason to justify your answer.

    1.1 Meaning of key ecological terms and biodiversity
    Activity 1.1
    1. What do you understand by the following terms: biodiversity, species, niche, population, and community?
    2. Differentiate between ecological niche and habitat.

    1.1.1 Key ecological terms
    Species is a group of closely related organisms which are capable of interbreeding to produce fertile offspring. Occasionally two organisms which are genetically closely related but not of the same species can interbreed to produce infertile offspring. For example:
    –– A cross between a donkey and a horse produces a mule, which is infertile. Thus, a donkey and a horse do not belong to the same species
    –– Lions and tigers belonging to different species. However, when a male tiger mates with a female lion they can have fertile offspring called tiglons, although the offspring of female tigers and male lions called ligers are not fertile

    Note that normally, tigers are forest dwellers and lions are plains dwellers and they are ecologically isolated. Breeding has only been observed in captivity. An ecological population is a group of individuals of the same species which live in a particular area at any given time.

    An ecological community consists of populations of different species which live in the same place at the same time, and interact with each other. A habitat is a specific area or place in which an individual organism lives. When a habitat is very small it is regarded as a microhabitat. Most ecosystems contain several habitats, and one species can have more than one habitat constituting its geographic range.

    An ecological niche is the status or the role of an organism in its habitat or the mode of life of an organism within its habitats. For example, insects are pollinating agents and preys of insectivores.

    Abiotic factor are non-living physical aspects of the environment such as the sunlight, soil, temperature, wind, water, and air. Biotic factors are the living organisms in the environment. They include organisms and their interactions with each other.

    An ecosystem is a natural unit consisting of biotic and abiotic factors through which energy flows and nutrients recycle. In an ecosystem, nutrients pass between different organisms in definite pathways. For example, nutrients in the soil are taken up by plants, which are then eaten by herbivores, which in turn may be eaten by carnivores and recycled by decomposers.

    A biome is a group of ecosystems that have the same climate and similar dominant communities. The highest level of organization is the entire biosphere.

    The Biosphere is the whole of the earth’s surface, the sea and the air that is inhabited by living organisms. The biosphere is made up of all ecosystems.

    1.1.2. Biodiversity

    Biodiversity is defined as the full range of variety and variability within and among living organisms and the ecological complexes in which they occur.

    Self-assessment 1.1
    1. Describe the two main components of an ecosystem.
    2. Hippopotamus has different habitats. It was found that the resting habitat is different from the mating habitat, and these two habitats are different from the area where this animal gets food. Explain the ecological term given to this set of habitats.

    1.2 Identification of biodiversity
    Activity 1.2

    Use books or other sources of information to answer the followings questions:
    1. What kinds of initiatives and incentive mechanisms are put in place by the Government of Rwanda to motivate local community in biodiversity conservation?
    2. Describe different ways used to identify biodiversity.
    3. Discuss the values of biodiversity and ecosystem services in Rwanda.
    4. Evaluate the contribution of biodiversity to human well-being.

    1.2.1. Categories of biodiversity
    Biodiversity can be categorized into three groups:
    –– Genetic diversity: the combination of different genes found within a population of a single species, and the patterns of variation found within different populations of the same species.
    –– Species diversity: the variety and abundance of different types of organisms which inhabit an area.
    –– Ecosystem diversity: the variety of habitats that occur within a region, or within the mosaic of patches found within a landscape.

    1.2.2. Importance of biodiversity
    Biodiversity contributes to ecosystem goods and services. The ecosystem goods and services include:
    –– Provision of food, air, fire wood, medicines(Fig.1.2), energy, fresh water.
    –– Nutrient cycling such carbon, water and nitrogen cycles by microorganisms and primary production by photosynthesis.
    –– Cultural or aesthetic service recreation, ecotourism, cultural andreligious inspiration.

    1.2.3. The threats and consequences of biodiversity loss Causes of biodiversity loss

    The main causes of biodiversity loss can be attributed to the influence of human activities on ecosystems. Threats to biodiversity include:

    a. Habitat loss and the degradation of the environment

    The habitat loss and the degradation of the environment occur in different ways.
    The most occurring, are tree cutting, agriculture and fires (Figure1.3). These human activities lead to the alteration and loss of suitable habitats for biodiversity. As a consequence, there is a loss of plant species as well as the decrease in the animal species associated to this plant diversity.

    b. Introduction of invasive alien species and genetically modified organisms

    Species originating from a particular area are harmful to native species also called endemic species when they are introduced into new natural environments. They can lead to different forms of imbalance in the ecological equilibrium, so that endemic species may fail to compete with introduced species, and they may affect the abundance and distribution in natural habitat.

    c. Pollution
    Human activities such as excessive use of fertilizers, and increased pollutants from industries and domestic sewage affect biodiversity. They contribute to the alteration of the flow of energy, chemicals and physical constituents of the environment and hence species may die as a result of toxic accumulation.

    d. Overexploitation of natural resources
    Increased hunting, fishing, and farming in particular areas lead to the decrease and loss of biodiversity due to excessive and continuous harvesting without leaving enough time for the organisms to reproduce and stabilize in their natural habitat.

    e. Climate change
    This is a change in the pattern of weather, related changes in oceans, land surfaces and ice sheets due to global warming resulting from man’s activities. Increasing global temperatures have resulted into melting of icebergs raising sea levels and so flooding coastal areas eventually affecting the niche. Consequences of loss of biodiversity
    They are various consequences of loss of biodiversity that include:
    –– Desertification, is thought by scientists to be a consequence of climate change, has been considered to be related to deforestation. Disrupting water cycles and soil structure results into less rainfall in an area.
    –– Floods as a result of rising sea levels
    –– Habitat destruction for extensive farming, timber harvesting and infrastructure and settlement
    –– Decrease in food production as result of change in pattern of weather that affects productivity
    –– Large scale deforestation has a negative effect on nutrient recycling and can accelerates soil erosion
    –– Diseases that come as effects of floods and malnutrition due to famine

    Self-assessment 1.2
    1. Define the term Extinction.
    2. Suggest the causes of extinction of species in Rwanda.
    3. Discuss the benefits of biodiversity to humans
    4. Discuss the major factors leading to the degradation of ecosystems in Rwanda
    5. Discuss the contribution of ecosystems to cultural traditions in Rwanda.
    6. In Rwanda different plants are used in traditional medicine to treat different diseases. Conduct a research and list at least 20 medicinal plants and the diseases they treat. From the list above describe at least one medicinal plant and get ready to present your work. The project work should include: written content of 2 pages in minimum and 4 pages in maximum, a testimony of people that have used plant species.
    7. Pollution is one of the causes of aquatic biodiversity loss.
    a. What do you understand by water pollution?
    b. Outline human activities that contribute to water pollution
    c. Discuss how polluted water affects aquatic living organisms?

    1.3 Calculation of Simpson’s index
    Activity 1.3

    A survey on tree species was conducted in Gako forest by a group of students. Five tree species (A to E) were identified and counted. The numbers found during this exercise are summarized in the following table:
    1. Describe the relative abundance of species A to E.
    2. Based on the data in the above table, suggest how species diversity of tree species can be calculated.
    There are many ways to measure diversity. The Simpson diversity index among indices used to measure diversity. It is expressed in three related indices namely Simpson index, Simpson index of diversity and Simpson reciprocal index.

    a. Simpson index D
    Simpson index D can be expressed in two ways and takes into consideration the total number of organisms of a particular species and the total number of organisms of all species. It is calculated as follows:with n: the total number of organisms of a particular species and N: the total number of organisms of all species. When the index equals or is nearby 0 there is an infinite diversity of considered species. When it equals or is nearby 1, this means that there is no diversity. The bigger the value of D, the lower the diversity and small is D, the bigger is the diversity.

    b. Simpson index of diversity 1 – DThe value of this index ranges between 0 and
    1, but now, the greater the value, the greater the sample diversity. This makes more sense. In this case, the index represents the probability that two individuals randomly selected from a sample will belong to different species.

    c. Simpson reciprocal index 1 / D
    Another way of overcoming the problem of the counter-intuitive nature of Simpson’s index is to take the Simpson’s reciprocal index 1 / D. The value of this index starts with 1 as the lowest possible figure. This figure would represent a community containing only one species. The higher is the value of Simpson reciprocal index, the greater the biological diversity.

    1. In woodland, a quadrat was sampled for ground vegetation. Data collected were recorded in the table 1.3.2. Find out the value of the Simpson index and draw the conclusion about the biological diversity of the sampled area.
    Table 1.3.2: Recorded data on the vegetation from a woodland
    Solution: Putting the figures into the formula for Simpson’s Index:

    Based on the meaning of Simpson index, the quadrat presents a low diversity because the value of D is near zero and zero and below 0.5.
    2. Calculate the value of Simpson’s Diversity Index (D) for a single quadrate sample of ground vegetation in woodland from which the following sampling date was obtained:

    Self-assessment 1.3
    1. Differentiate between species richness and species evenness
    2. Suggest precautions taken when measuring populations of aquatic animals or plants.
    3. Explain why a habitat with high diversity tends to be more stable than one with lower diversity.
    4. In a survey of trees in a tropical forest, students identified five tree species (A to E).
    They counted the numbers of trees in an area 100 m × 100 m and found these results:

    Calculate the Simpson’s Index diversity for identified species and explain the advantage of using data on species diversity and abundance when calculating an index of diversity.

    5. The Simpson’s Index of diversity for vegetation in an open area inhabited by grasslands was 0.8. For a similar sized area of vegetation beneath some conifer trees it was 0.2. What do you conclude from these results?

    1.4 Sampling techniques to assess the distribution and abundance of organisms
    Activity 1.4

    From your school garden, sample different flowering plant species and answer the following questions:
    1. Specify the techniques used for collecting flowers of different species.
    2. What are the advantages of the technique you used for data collection?
    3. Move around the school garden and collect different specimens of plant species. Name the collected species by using their names. In case you don’t know their names, use letters A, B, C ….
    Calculate Simpson index D, Simpson index of diversity and Simpson reciprocal index.

    To calculate Simpson’s index for a particular place:
    –– Identify the habitat to be studied.
    –– The number of individuals sampled for each species must be recorded.
    To analyze the distribution and abundance of organisms in an area of study, there are different sampling methods.
    Note that, sampling only one quadrat would not give reliable estimate of the diversity of the ground flora in the wood.

    a. Random sampling method
    A random sampling method is a sampling method where samples are taken from different positions within a habitat and those positions are chosen randomly.

    b. Quadrat sampling method
    A quadrat is a square area that is marked using a pre-made square of plastic, or stakes and string and it can range in size. Different species and their numbers within the quadrat are counted. Counting is repeated many times in different places in the habitat to get an accurate representation of biodiversity.

    c. Frame quadrats
    Frame quadrats are small plot used to isolate a standard unit of area for the study of the distribution of an item over a large area. While originally rectangular, modern quadrats can be rectangular, circular, and /or irregular. The quadrat is suitable for sampling plants, slow-moving animals such as millipedes and insect and some aquatic organisms.
    d. Transect sampling
    Transect sampling is done using a transect line, which is usually a rope or measuring tape that has been marked at set intervals, such as every meter. The line is unrolled within the habitat. At every interval, the type and number of species along the line are recorded. A measured line is laid across the area in the direction of the environmental gradient. The species touching the line can be recorded along the whole length of the line (continuous sampling) or at specific points along the line (systematic sampling).
    e. Belt transects method
    Belt transects method is the same as the line transects but widens the sampling area. The samples are taken and the abundance, percentage cover in a defined area determined. Samples can be taken within the belt.
    f. Netting
    Netting is a sampling method where fine mesh nets are used to capture different organisms that include insects, birds and bats. The technique is also used for sampling small aquatic organisms like daphnia, and water boatman.
    g. Capture -recapture technique
    This method is useful for sampling non-fixed population and is suitable for animal such as fishes, birds, lizards and insects. A sample of the population to be studied is first captured and each individual is marked with a spot for identification. These are then released and given enough time to mix up with the rest of the members in the habitat. After a certain period of time, another sample is taken. During the mark-release-recapture technique, the total population can be estimated by the use of the formula: where
    n1 is a number caught and marked in first sample,
    n2 is a number caught in second sample
    n3 is a number in the second sample that had been marked.
    To understand this application, let us use the following examples:

    1. A team of students used a sweep net to sample brown grasshoppers
    and each collect insect was marked with a very small spot of non-toxic waterproof paint and then they were released in the field. The next day, a second large sample was conducted and data were recorded as follows: number of caught and marked in first sample (n1) = 247, number of caught in second sample (n2) = 269, and the number in the second sample that had been marked (n3) = 16. What is the number of estimated population?
    2. A student collected 16 butterflies which he marked and released. For a second time he collected 18 butterflies among which 12 were already marked from the first sampling. Estimate the population size of butterflies in that area.
    Self-assessment 1.4
    1. Explain the advantages of the random sampling techniques.
    2. Use suitable methods, such as frame quadrats, line transects, and belt transects, to assess the distribution and abundance of insect species in a school garden. Record your data and use the Simpson index ofdiversity (D) to calculate the diversity of collected insects.
    3. Suggest the benefi ts of using the following sampling techniques:
    a. Quadrats
    b. Transect
    c. Mark-capture-recapture
    4. State the conditions in which quadrats, transect and mark recapture are
    suitable sampling methods.

    1.5 Pearson’s linear correlation
    Activity 1.5

    Some of the following fi gures indicate a positive, negative or non-correlation.

    1. What do you understand by the term correlation?
    2. Categorize the graphs given as positive, negative or weak or no correlation
    3. In which conditions results can indicate a positive correlation?
    4. Conclude about your results when there is no correlation.
    To decide if there is an association between collected data, a correlation coeffi cient is calculated and plot scatter graph drawn in order to make a judgment. The strongest correlation is present for studied items when all the points lie on a straight line. In this case, there is linear correlation, and the correlation coeffi cient equals

    1. If a given variable X increases so does another variable Y, the relationship is a positive correlation. If a variable X increases while the variable Y decreases, then the relationship is a negative correlation. A correlation coefficient of 0 means there is no correlation at all. These correlation coefficients are ways to test a relationship observed and recorded to see if the variables are correlated and, if so, to find the strength of that correlation.

    a. Pearson’s correlation coefficient
    Pearson’s correlation coefficient can only be used where there might be a linear correlation and when there are collected quantitative data as measurements (for example, length, height, depth, and light intensity, mass) or counts (for example number of plant species in quadrats). The data must be normally distributed.
    r               is the correlation coefficient
    x              is the number of species in a quadrat
    y              is the number of species in the same quadrat
    n              is the number of readings (From1 to n)
    x              is the mean number of species
    y              is the mean number of species
    sx                  is the standard deviation for x
    sy                  is the standard deviation for y

    Self-assessment 1.5
    Use Pearson’s linear correlation to analyze the relationships between the distribution and abundance of species and abiotic or biotic factors.

    End of unit assessment 1

    Section A: Answer as true or false
    1. Abiotic factors are the non-living physical aspects of the environment.
    2. Capture –recapture is a method used to integrate the numbers of mobile animals in a particular place.
    3. A correlation coefficient of 0 means that there is no correlation at all.
    4. A sample is a portion, piece, or segment that is representative of a whole area of study.
    5. In the Simpson’s index, Nrepresentsthetotal number of organisms of a particular species

    Section B: Long and short answer based questions
    1. What do you understand by the term biodiversity?
    2. What do you think would happen to plants if there were no insects?
    3. Suggest different ways to conserve our forests.
    4. A student has randomly collected 5 types of species at the following frequencies.
    Calculate the Simpson’s diversity index of this community.
    5. A team of students conducted the capture- recapture sampling method of tilapia from lake Muhazi at different times of the day as recorded in the data below:
    a. Plot the graph for the date provided and describe the shape of the graph.
    b. From the graph, determine the appropriate time to have the most catch.
    6. What do you understand by term endangered species?
    7. Describe how diversity is threatened by climate change and human activities.